核酸適配體在真菌毒素檢測中研究進展

<p>&nbsp;</p> <p><strong>【摘 要】</strong>核酸適配體是指在體外利用指數富集的配基系統進化技術篩選得到的核酸（DNA 或 RNA）片段。基于核酸適配體的檢測方法具有特異性高、穩定性好、易于修飾等優點，在真菌毒素檢測領域得到了廣泛的關注和應用。本文在傳統檢測方法的基礎上，著重介紹核酸適配體在真菌毒素檢測領域中的研究進展，并提出目前存在的問題及發展趨勢。&nbsp;</p> <p><img src="/images/upload/Image/4444(1).png" width="694" height="637" alt="" /></p> <p><b>&ldquo;上海酶聯&rdquo;&nbsp;</b><strong>參考文獻</strong></p> <p>[ 1 ] 張思思, 陸繼偉, 王少敏,等. 國內外真菌毒素檢測方法研究現狀及進展[J]. 食品安全質量檢測學報, 2016, 7(7): 2575 &ndash; 2586.</p> <p>[ 2 ] International Agency for Research on Cancer. IARC monographs on the evaluation of carcinogenic risks to humans, 2015 [EB/OL]. Available from: http://monographs.Iarc.fr/ENG/Classification/ latest_classif.php.</p> <p>[ 3 ] 王元凱, 王君, 嚴亞賢. 玉米赤霉烯酮檢測方法研究進展[J]. 中國公共衛生, 2009, 25(9): 1100 &ndash; 1101.</p> <p>[ 4 ] 李蓉, 黃瑩偲, 王勇,等. 食品中真菌毒素檢測技術的研究進展[J].中國衛生檢驗雜志, 2015, 25(18): 3195 &ndash; 3198.</p> <p>[ 5 ] Jutamart K, Saranya P, Supaporn I, et al. Simultaneous detection of multiple mycotoxins in broiler feeds using a liquid chromatography tandem-mass spectrometry[J]. Journal of Veterinary Medical Science, 2015, 78(2): 259 &ndash; 264.</p> <p>[ 6 ] Tuerk C, Gold L. Systematic evolution of ligands by exponential enrichment: RNA ligands to bacteriophage T4 DNA polymerase[J]. Science, 1990, 249(4968): 505 &ndash; 510.</p> <p>[ 7 ] Ellington AD, Szostak JW. In vitro selection of RNA molecules that bind specific ligands[J]. Nature, 1990, 346(6287): 818 &ndash; 822.</p> <p>[ 8 ] Y&uuml;ce M, Ullah N, Budak H. Trends in aptamer selection methods and applications[J]. Analyst, 2015, 140(16): 5379 &ndash; 5399.</p> <p>[ 9 ] Cruzaguado JA, Penner G. Determination of ochratoxin a with a DNA aptamer[J]. Journal of Agricultural and Food Chemistry, 2017, 56(22): 10456 &ndash; 10461.</p> <p>[ 10 ] Mckeague M, Velu R, Hill K, et al. Selection and characterization of a novel DNA aptamer for label-free fluorescence biosensing of ochratoxin A[J]. Toxins, 2014, 6(8): 2435 &ndash; 2452.</p> <p>[ 11 ] Barthelmebs L, Jonca J, Hayat A, et al. Enzyme-linked aptamer assays (ELAAs), based on a competition format for a rapid and sensitive detection of ochratoxin A in wine[J]. Food Control, 2011, 22(5): 737 &ndash; 743.</p> <p>[ 12 ] Le LC, Cruz-Aguado JA, Penner GA. Ligands for aflatoxin and zearalenone: US, WO/2011/020198[P]. 2011.</p> <p>[ 13 ] Ma X, Wang W, Chen X, et al. Selection, identification, and application of aflatoxin B1 aptamer[J]. European Food Research and Technology, 2014, 238(6): 919 &ndash; 925.</p> <p>[ 14 ] Setlem K, Mondal B, Ramlal S, et al. Immuno affinity SELEX for simple, rapid, and cost-effective aptamer enrichment and identification against aflatoxin B1[J]. Frontiers in Microbiology, 2016, 7: 1909.</p> <p>[ 15 ] Ma X, Wang W, Chen X, et al. Selection, characterization and application of aptamers targeted to aflatoxin B2[J]. Food Control, 2015, 47: 545 &ndash; 551.</p> <p>[ 16 ] Malhotra S, Pandey AK, Rajput YS, et al. Selection of aptamers for aflatoxin M1 and their characterization[J]. Journal of Molecular Recognition, 2014, 27(8): 493 &ndash; 500.</p> <p>[ 17 ] Mckeague M, Bradley CR, Girolamo AD, et al. Screening and initial binding assessment of fumonisin B1 aptamers[J]. International Journal of Molecular Sciences, 2010, 11(12): 4864 &ndash; 4881.</p> <p>[ 18 ] Chen X, Huang Y, Duan N, et al. Selection and characterization of single stranded DNA aptamers recognizing fumonisin B1[J]. Microchimica Acta, 2014, 181(11): 1317 &ndash; 1324.</p> <p>[ 19 ] Frost NR, Mckeague M, Falcioni D, et al. An in solution assay for interrogation of affinity and rational minimer design for small molecule-binding aptamers[J]. Analyst, 2015, 140(19): 6643 &ndash; 6651.</p> <p>[ 20 ] Chen XJ, Huang YK, Duan N, et al. Selection and identification of ssDNA aptamers recognizing zearalenone[J]. Analytical and Bioanalytical Chemistry, 2013, 405(20): 6573 &ndash; 6581.</p> <p>[ 21 ] Chen XJ, Huang YK, Duan N, et al. Screening and identification of DNA aptamers against T-2 toxin assisted by graphene</p> <p>&nbsp;</p>